您当前的位置: 产品中心>
Medimachine II 组织匀浆及单细胞悬液制备仪


技术原理

         Medimachine II 采用机械剪切的原理,根据用户的不同需要和技术特性,选择适当的一次性耗材,配合相应的操作程序,实现对于组织样品的温和分离或精细均质化。

  • Medimachine II主机具有两个马达:低速马达最高转速为90rpm,高速马达最高转速为22200rpm。
  • Medicons / Medicons Max 组织分离器内嵌固定的不锈钢网筛,其上分布有六角形筛孔,每个筛孔周围有6个经特殊设计的微型刀片以进行精细的组织切割,与主机的低速马达配合可用于动物和植物组织细胞的分离以及从生物实体组织中制备单细胞悬液。
  • 细胞悬液经不同孔径的 Filcons / Filcons Max 过滤器过滤,完成细胞分选和纯化,用于后续的流式细胞分析及细胞培养等相关研究与操作。
  • FOODcon7 / Prypcons 匀质杯配合主机的高速马达使用,快速制备样品匀浆,实现组织和细胞的精细均质化。




产品应用/特点


Medimachine II 应用

        流式细胞术(flow cytometry,FCM)作为一种可在单细胞水平上对大量细胞进行快速、准确、多参数定量分析和分选的技术,在细胞生物学、免疫学、肿瘤学以及临床诊断分析等方面有着广泛而深入的应用。进行FCM分析的前提是单细胞悬液样品的制备,此外,单细胞悬液也是获得细胞分离纯化、细胞分析以及细胞培养可靠结果的关键先决条件。
          Medimachine II,作为已在生物科研领域被广泛使用的Medimachine System的全新升级产品,可在30s~4min内完成实体组织单细胞悬液样品的制备,组织细胞的分离过程自动完成,样品悬液中活细胞比例高(cell viability,在程序正确完成的情况下可达到80%),适合进行FCM分析、活体组织检查以及相关细胞的筛选、纯化和培养。
         此外,Medimachine II还具备对生物样品的精细均质化功能,产物可用于核酸提取、DNA扩增等分子生物学研究以及化学残留物、病毒和细菌的检测分析等。

单细胞悬液制备&精细匀质--双重模式

         Medimachine II将生物样品的单细胞悬液制备与均质化处理两种功能整合于一体,极大地方便了用户的不同需求。


核心应用

·流式细胞分析
·细胞分离与纯化
·细胞培养
·活体组织检查
·核酸提取与DNA扩增
·化学残留物、病毒和细菌分析等

性能特点

·具有交互式触摸屏,可戴实验室手套触控操作。
·软件提供有操作模式和操作咨询功能,并有多种语言可供设置。
·具有手动和自动两种模式:手动模式下,可自主设定运行时间;自动模式下,直接选定时间选项。
·具有两个马达:低速马达最高转速为90rpm,实现单细胞分离;高速马达最高转速为22,200rpm,实现组织均质化。
·程序运行结束时,马达会自动停止,上盖自动解锁。
·程序运行期间,用户根据需要可以控制终止运行。
·用户可根据需要添加或删除新的自定义程序。
·具有多种颜色的机身效果可供设置。


耗材

 

Medicons组织分离器(1 ml)

·无菌包装,35μm筛孔孔径
·无菌包装,50μm筛孔孔径
·普通包装,35μm筛孔孔径
·普通包装,50μm筛孔孔径


Medicons Max组织分离器(16 ml )

·无菌包装,50μm筛孔孔径
·普通包装,50μm筛孔孔径

 


Filcons细胞过滤器(滤膜面积80mm2)

-可与5ml Falcon离心管配套使用,离心管连接于流式细胞仪上,执行后续操作。

·无菌包装
·10μm滤膜孔径(棕)
·20μm滤膜孔径(红)
·30μm滤膜孔径(橙)
·50μm滤膜孔径(绿)
·70μm滤膜孔径(紫)
·100μm滤膜孔径(黑)
·200μm滤膜孔径(白)
·500μm滤膜孔径(蓝)

·杯型(适用5ml以下细胞悬液的过滤)
·10μm滤膜孔径(棕)
·20μm滤膜孔径(红)
·30μm滤膜孔径(橙)
·50μm滤膜孔径(绿)
·70μm滤膜孔径(紫)
·100μm滤膜孔径(黑)
·200μm滤膜孔径(白)
·500μm滤膜孔径(蓝)

·普通包装
·柱型(适用5ml以上细胞悬液的过滤)
·10μm滤膜孔径(棕)
·20μm滤膜孔径(红)
·30μm滤膜孔径(橙)
·50μm滤膜孔径(绿)
·70μm滤膜孔径(紫)
·100μm滤膜孔径(黑)
·200μm滤膜孔径(白)
·500μm滤膜孔径(蓝)

·杯型(适用5ml以下细胞悬液的过滤)
·10μm滤膜孔径(棕)
·20μm滤膜孔径(红)
·30μm滤膜孔径(橙)
·50μm滤膜孔径(绿)
·70μm滤膜孔径(紫)
·100μm滤膜孔径(黑)
·200μm滤膜孔径(白)
·500μm滤膜孔径(蓝)

 


Filcons Max细胞过滤器(滤膜面积660 mm2)

-可与50ml Falcon锥形离心管或同类型50ml离心管配套使用。

·无菌包装
·杯型
·30μm滤膜孔径(橙)
·50μm滤膜孔径(绿)
·70μm滤膜孔径(紫)
·100μm滤膜孔径(黑)

·普通包装
·杯型
·30μm滤膜孔径(橙)
·50μm滤膜孔径(绿)
·70μm滤膜孔径(紫)
·100μm滤膜孔径(黑)

 


FOODcon匀质样品杯

·最多可加入25?ml样品

 


Prypcons匀质样品杯

·最多可加入6 ml样品






产品规格

型号

Medimachine II

认证标准

CE认证;符合UL / CSA

尺寸

长×宽×高

211 mm x 164 mm x 257 mm

重量

2.50 kg

主电源

功耗

120W

电源电压

85~264 VAC, 47-63 Hz

保险丝

2 x 2A

马达

低速规格

转速 - > 90 rpm

旋转方向 - > 顺时针

最大扭矩 - > 420 mNm

高速规格

转速 - > 22,200 rpm

旋转方向 - > 顺时针

失速转矩 - > 123 mNm

操作条件

工作温度

+5到+45℃

湿度

低于90%

海拔高度

无要求

储存温度

0 到+50°C

参考文献 


·Schreurs RRCE, Drewniak A, Bakx R. Quantitative comparison of human intestinal mononuclear leukocyte isolation techniques for flow cytometric analyses[J]. J Immunol Methods. 2017, 445:45-52.
·Schneider F, Redmann K, Wistuba J. Comparison of enzymatic digestion and mechanical dissociation of human testicular tissues[J]. Fertil Steril. 2015, 104(2):302-11.
·Pereira LE, Makarova N, Dobard C. Development and optimization of a non-enzymatic method of leukocyte isolation from macaque tissues[J]. J Med Primatol. 2014, 43(5):360-3.
·Rodríguez-Casuriaga R, Geisinger A, López-Carro B. Ultra-fast and optimized method for the preparation of rodent testicular cells for flow cytometric analysis[J]. Biol Proced Online. 2009, 11:184-95.
·Novotny JR, Schmücker U, Staats B. Failed or inadequate bone marrow aspiration: a fast, simple and cost-effective method to produce a cell suspension from a core biopsy specimen[J]. Clin Lab Haematol. 2005, 27(1):33-40.
·Molnar B, Bocsi J, Karman J. Immediate DNA ploidy analysis of gastrointestinal biopsies taken by endoscopy using a mechanical dissociation device[J]. Anticancer Res. 2003, 23(1B):655-60.
·Novelli M, Savoia P, Cambieri I. Collagenase digestion and mechanical disaggregation as a method to extract and immunophenotype tumour lymphocytes in cutaneous T-cell lymphomas[J]. Clin Exp Dermatol. 2000, 25(5):423-31.
·Brockhoff G, Fleischmann S, Meier A. Use of a mechanical dissociation device to improve standardization of flow cytometric cytokeratin DNA measurements of colon carcinomas[J]. Cytometry. 1999, 38(4):184-91.
·Ottesen GL, Christensen IJ, Larsen JK. Tissue disaggregation for flow cytometric DNA analysis: comparison of fine-needle aspiration and an automated mechanical procedure[J]. Cytometry. 1996, 26(1):65-8.
·Marie Violet, Lucie Delattre, Meryem Tardivel. A major role for Tau in neuronal DNA and RNA protection in vivo under physiological and hyperthermic conditions[J]. Front Cell Neurosci. 2014, 8: 84.
·Frank Liang, Aurélie Ploquin, José DelaO Hernández. Dissociation of Skeletal Muscle for Flow Cytometric Characterization of Immune Cells in Macaques[J]. J Immunol Methods. 2015, 425: 69-78.
·Ana C. Lima, Min Jung, Jannette Rusch. Multispecies Purification of Testicular Germ Cells[J]. Biol Reprod. 2016, 95(4): 85.
·Raquel López-Fontal, Miriam Zeini, Paqui G. Través. Mice Lacking Thyroid Hormone Receptor β Show Enhanced Apoptosis and Delayed Liver Commitment for Proliferation after Partial Hepatectomy[J]. PLoS One. 2010, 5(1): e8710.
·Maria R Parkhurst, James C Yang, Russell C Langan. T Cells Targeting Carcinoembryonic Antigen Can Mediate Regression of Metastatic Colorectal Cancer but Induce Severe Transient Colitis[J]. Mol Ther. 2011, 19(3): 620-626.
·Bjoern Gaertner, Jeff Johnston, Kai Chen. Poised RNA Polymerase II changes over developmental time and prepares genes for future expression[J]. Cell Rep. 2012, 2(6): 1670-1683.
·Karan M Shah, Matt M Stern, Amber R Stern. Osteocyte isolation and culture methods[J]. Bonekey Rep. 2016, 5: 838.
·G. L. Ottesen, I. J. Christensen, J. K. Larsen. DNA aneuploidy in early breast cancer[J]. Br J Cancer. 1995, 72(4): 832-839.
·Zhenjiang Liu, Qingda Meng, Jiri Bartek. Tumor-infiltrating lymphocytes (TILs) from patients with glioma[J]. Oncoimmunology. 2017, 6(2): e1252894.
·Benjamin D. Moore, Ramon U. Jin, Luciana Osaki. Identification of alanyl aminopeptidase (CD13) as a surface marker for isolation of mature gastric zymogenic chief cells[J]. Am J Physiol Gastrointest Liver Physiol. 2015, 309(12): G955-G964.
·Henry Radziewicz, Chris C. Ibegbu, Huiming Hon. Transient CD86 expression on HCV specific CD8+ T cells in acute infection is linked to sufficient IL-2 signaling[J]. J Immunol. 2010, 184(5): 2410-2422.
·Anna L Olsen, Joanne M Davies, Louise Medley. Quantitative Analysis of Survivin Protein Expression and Its Therapeutic Depletion by an Antisense Oligonucleotide in Human Lung Tumors[J]. Mol Ther Nucleic Acids. 2012, 1(6): e30.
·Zhenjiang Liu, Martin Rao, Thomas Poiret. Mesothelin as a novel biomarker and immunotherapeutic target in human glioblastoma[J]. Oncotarget. 2017, 8(46): 80208-80222.
·Ingrid Olsen, Stig Tollefsen, Claus Aagaard. Isolation of Mycobacterium avium Subspecies paratuberculosis Reactive CD4 T Cells from Intestinal Biopsies of Crohn's Disease Patients[J]. PLoS ONE. 2009, 4(5): e5641.
·Sally Spendiff, Mojgan Reza, Julie L. Murphy. Mitochondrial DNA deletions in muscle satellite cells: implications for therapies[J]. Hum Mol Genet. 2013, 22(23): 4739-4747.
·Linh T. Nguyen, Pei Hua Yen, Jessica Nie. Expansion and Characterization of Human Melanoma Tumor-Infiltrating Lymphocytes (TILs)[J]. PLoS One. 2010, 5(11): e13940.
·Aleena A. Gladkikh, Daria M. Potashnikova, Victor Tatarskiy. Comparison of the mRNA expression profile of B‐cell receptor components in normal CD5‐high B‐lymphocytes and chronic lymphocytic leukemia: a key role of ZAP70[J]. Cancer Med. 2017, 6(12): 2984-2997.
·Vanessa Carregaro, Diego Luis Costa, Claudia Brodskyn. Dual effect of Lutzomyia longipalpis saliva on Leishmania braziliensisinfection is mediated by distinct saliva-induced cellular recruitment into BALB/c mice ear[J]. BMC Microbiol. 2013, 13: 102.
·Steven A. Rosenberg, Mark E. Dudley. Cancer regression in patients with metastatic melanoma after the transfer of autologous antitumor lymphocytes[J]. Proc Natl Acad Sci U S A. 2004, 101(Suppl 2): 14639-14645.
·Liping Nie, Ana E. Vázquez, Ebenezer N. Yamoah. Identification of Transcription Factor–DNA Interactions Using Chromatin Immunoprecipitation Assays[J]. Methods Mol Biol. 2009, 493: 311.
·Henry Radziewicz, Chris C. Ibegbu, Marina L. Fernandez. Liver-Infiltrating Lymphocytes in Chronic Human Hepatitis C Virus Infection Display an Exhausted Phenotype with High Levels of PD-1 and Low Levels of CD127 Expression[J]. J Virol.2007, 81(6): 2545-2553.
·Andreas Krieg, Sabrina Mersch, Inga Boeck. New Model for Gastroenteropancreatic Large-Cell Neuroendocrine Carcinoma: Establishment of Two Clinically Relevant Cell Lines[J]. PLoS One. 2014, 9(2): e88713.
·Beno t Callendret, Jens Bukh, Heather B. Eccleston. Transmission of Clonal Hepatitis C Virus Genomes Reveals the Dominant but Transitory Role of CD8+T Cells in Early Viral Evolution[J]. J Virol. 2011, 85(22): 11833-11845.
·Tao Luo, Shijun Cui, Chunjing Bian. Crosstalk between TGF-β/Smad3 and BMP/BMPR2 signaling pathways via miR-17~92 cluster in carotid artery restenosis[J]. Mol Cell Biochem. 2014, 389(0): 169-176.
·Kai-Hui Chan, Sebastian P Galuska, Pradeep Kumar Kudipudi. Signaling by TGF-betas in tubule cultures of adult rat testis[J]. Am J Transl Res.2017, 9(3): 1173-1182.
·Najam Ali Khan, M. Abid, Aftab Ahmad. Cardioprotective Effect of Coenzyme Q10 on Apoptotic Myocardial Cell Death by Regulation of Bcl-2 Gene Expression[J]. J Pharmacol Pharmacother. 2017, 8(3): 122-127.
·S Derniame, J-M Vignaud, G C Faure. Alteration of the immunological synapse in lung cancer: a microenvironmental approach[J]. Clin Exp Immunol. 2008, 154(1): 48-55.
·Marc McClelland, Liujian Zhao, Shannon Carskadon. Expression of CD74, the Receptor for Macrophage Migration Inhibitory Factor, in Non-Small Cell Lung Cancer[J]. Am J Pathol. 2009, 174(2): 638-646.
·Mehdi Rashighi, Priti Agarwal, Jillian M Richmond. CXCL10 is critical for the progression and maintenance of depigmentation in a mouse model of vitiligo[J]. Sci Transl Med. 2014, 6(223): 223ra23.
·Irene da Cruz, Rosana Rodríguez-Casuriaga, Federico F. Santi aque. Transcriptome analysis of highly purified mouse spermatogenic cell populations: gene expression signatures switch from meiotic-to postmeiotic-related processes at pachytene stage[J]. BMC Genomics. 2016, 17: 294.

·Nerys D Forester, Sheena M Cruickshank, D Julian A Scott. Functional characterization of T cells in abdominal aortic aneurysms[J]. Immunology.2005, 115(2): 262–270.


Medimachine II产品彩页(博益)


  • 扫码获取相关资讯
  • 扫码关注公众号

北京博益伟业仪器有限公司

办公地址: 北京市朝阳区立水桥北里2号塞纳维拉2509

邮编:100012

电话:010-58294669,010-64842355,010-64842356

邮箱:sales@bio-one.cn

版权所有©北京博益伟业仪器有限公司京ICP备05041067号